1. fixation: place in cassette, fix in formalin 2. tissue processing (tissue is embedded in paraffin to prep for sectioning) -dehydration: run in automatic tissue processor overnight in series of baths of increasing concentrations of alcohol -clearing: remove dehydrant with xylene bath -replace xylene with paraffin -embedding: fill mold with paraffin, place tissue on mold, cool mold to form untrimmed tissue block 3. sectioning: use microtome (tomorrow) 4. staining (tomorrow)
1. fixation: place in cassette, fix in formalin
ReplyDelete2. tissue processing (tissue is embedded in paraffin to prep for sectioning)
-dehydration: run in automatic tissue processor overnight in series of baths of increasing concentrations of alcohol
-clearing: remove dehydrant with xylene bath
-replace xylene with paraffin
-embedding: fill mold with paraffin, place tissue on mold, cool mold to form untrimmed tissue block
3. sectioning: use microtome (tomorrow)
4. staining (tomorrow)