http://www.piercenet.com/browse.cfm?fldID=FA97D803-6953-48E4-A7BD-6947D35FE83B
Basic Protocol:
Mix:
Lysis Buffer
Protease Inhibitor (comes in pellet --> vortex with buffer)
Phosphorase Inhibitor
add to pulverized tumor samples
homogenize while keeping tube on ice
spin down using vortex
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