cDNA synthesis

Background Info:

http://dwb4.unl.edu/Chem/CHEM869N/CHEM869NLinks/www.dur.ac.uk/~dbl0www/Staff/Croy/cDNAfigs.htm

mix:

water
reaction mix
RNA
enzyme (keep on ice)

add RNA
tap it

PCR

Protein Extraction

http://www.piercenet.com/browse.cfm?fldID=FA97D803-6953-48E4-A7BD-6947D35FE83B

Basic Protocol:

Mix:
Lysis Buffer
Protease Inhibitor (comes in pellet --> vortex with buffer)
Phosphorase Inhibitor

add to pulverized tumor samples
homogenize while keeping tube on ice
spin down using vortex

Protein Assay- for measuring concentration

http://en.wikipedia.org/wiki/Bradford_protein_assay

FGFR2 - alternative pathway for angiogenesis

http://www.ncbi.nlm.nih.gov/omim/176943

http://www.fasebj.org/cgi/content/full/18/2/326

Taqman

http://www.diabetesgenome.org/docs/Patti_Lab_Real-Time_qPCR_pr.pdf

Oral Dosing

http://www.iacuc.ucsf.edu/Policies/awSPOralGavageMice.asp

BLAST Algorithm and Substitution Matrix

http://en.wikipedia.org/wiki/BLAST#Algorithm
http://en.wikipedia.org/wiki/Substitution_matrix