cell culture/passing

medium: 10%FBS in 1x AA, warm up for 1 hour before use

pass 1 to 4 in petri dish when crowded (twice a week):
vacuum out old medium, add 4mL trypsin-EDTA, incubate 3 minutes until cells float to top, add 4mL medium.
centrifuge 5 minutes, vacuum out supernatant, add 4mL medium to cells, mix thoroughly.
Add 1mL to each of 4 new petri dishes.
add medium to make total of 5mL solution if transferring to small petri dishes, 25mL if transferring to large petri dishes.

antibody for measuring cell death/dna fragmentation

http://en.wikipedia.org/wiki/Caspase_3

Batch Effect/Statistics stuff

Batch Effect
http://www.itl.nist.gov/div898/handbook/pmd/section6/pmd622.htm
http://www.goldenhelix.com/SNP_Variation/tutorials/cnv/batch-effects.html

PCA
http://en.wikipedia.org/wiki/Principal_component_analysis
http://www.youtube.com/watch?v=jqbqr5enSOM&feature=related

Animation of Microarray

http://www.bio.davidson.edu/courses/genomics/chip/chip.html

Antibody labeling- background on proteins

Ki-67
http://en.wikipedia.org/wiki/Ki-67_%28protein%29

CD31
http://en.wikipedia.org/wiki/CD31

Impressive Resume

http://www.scribd.com/doc/16652848/Biotechnology-Fresh-Graduate-Resume-Sandeep

Flow Cytometry

http://en.wikipedia.org/wiki/Flow_cytometry
http://info.med.yale.edu/immuno/cytometry/docs/pdf/introduction.pdf